Cspb promoter
WebJan 1, 2000 · The cspB promoter (PcspB) described by Peyret et al. (1993) was isolated as a. 554 bp Eco RI \ Eco NI fragment and ligated to an Eco NI \ Bam HI adaptor constructed with the two synthetic oligo- WebMay 9, 2009 · We used α-amylase from Streptococcus bovis 148 (AmyA) as a model protein to be displayed on the C. glutamicum cell surface. AmyA was fused to the C terminus of the porins PorB, PorC, or PorH. Expression vectors using fused proteins under the control of the cspB promoter were constructed and introduced into the C. glutamicum Cm strain.
Cspb promoter
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WebApr 1, 2006 · Furthermore, the promoter of the cspB gene was mapped by 5′ rapid amplification of cDNA ends PCR and the corresponding DNA fragment was used in DNA affinity purification assays. A 30 kDa protein specifically binding to the promoter region of the cspB gene was purified. Matrix-assisted laser desorption ionization time-of-flight … WebcspB promoter was constructed by cross-over PCR as described previously (Kikuchi et al. 2006). First-round PCR amplified the cspB promoter using Primer A (Csp5K, forward primer, Table 2) and Primer B (Csp-PPPg, reverse primer) and pPSPTG11 DNA as a template; the pro-PG gene with its own signal sequence was also amplified using
WebThe synthetic promoter library covered a wide range of strengths, ranging from 1 to 193% of the tac promoter. 68 promoters were selected and sequenced for correlation analysis between promoter ... WebThe major cold shock protein of Bacillus subtilis CspB binds with high affinity to the ATTGG- and CCAAT sequences in single stranded oligonucleotides ... a cis-element found in the promoter region of mammalian major histocompatability complex class II genes, which has the consensus sequence CTGATTGGC/TC/TAA [4,5]. The underlined penta-
WebSep 22, 2007 · Corynebacterium glutamicum is an important microorganism in the industrial production of amino acids. We engineered a strain of C. glutamicum that secretes α … WebDownload scientific diagram Genetic features of the cspB promoter region of C. glutamicum ATCC 14067 and visualization by SDS-PAGE of proteins binding in front of the cspB gene. (a) The ...
WebNov 1, 1992 · The DNA sequence of the cspB promoter region is shown at the bottom. Interruption of the chromosomal copy of the cspB gene by a double-crossover recombination event.
WebJan 1, 2008 · The promoter and signal sequence of the cspB gene encoding PS2, the major protein secreted by the organism, have been used in many studies of heterologous protein secretion in C. glutamicum [43,44 ... how to subtract spectra from spectraWebThe coding and promoter regions of the cspB gene encoding PS2 were cloned in lambda gt11 using polyclonal antibodies raised against PS2 for screening. Expression of the … how to subtract text from a cell in excelWebDec 1, 2011 · DNA fragments containing the promoter sequence of CspB from C. glutamicum and the signal sequence of CspA from C. ammoniagenes were fused to the CspB50TEV-Teriparatide gene. This synthesized construct was then inserted into the pPK4 plasmid and incorporated into the YDK010 bacterial strain, which is a derivative of wild … reading music sheets for beginnersWebSep 4, 2003 · Synthesis of CspB is high when these strains are grown on lactate, acetate, gluconate and tartrate but low when the carbon sources are glucose, fructose, sucrose … reading music notes worksheetWebunder the control of the cspB promoter were constructed and introduced into the C. glutamicum Cm strain. Immu-nostaining microscopy and flow cytometric analysis revealed that PorB-AmyA, PorC-AmyA, and PorH-AmyA were displayed on the C. glutamicum cell surface. AmyA activity was only detected in the cell fraction of C. reading music on pianoWebCSPB may refer to the following: California State Personnel Board, one of California's state constitutional offices; Credit Suisse Private Banking, a financial services company in … how to subtract string in c++Webadvantage of this cellular machinery, the CspB promoter and signal peptide of C. glutamicum can be used to synthesize and secrete soluble, properly folded, biologically active recombinant proteins directly into the growth medium with high purity. Since the two secretory pathways were elucidated, about 154 signal peptides have been screened. reading my contact lens prescription