WebApplications Tested: This 150D/E4 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that WebFOXP3 Monoclonal Antibody (FJK-16s), APC, eBioscience™ Advanced Verification This Antibody was verified by Relative expression to ensure that the antibody binds to the … Invitrogen Anti-FOXP3 Monoclonal (FJK-16s), eBioscience™, Catalog # 56-5773 … Invitrogen Anti-FOXP3 Monoclonal (FJK-16s), eBioscience™, Catalog # 45-5773 …
FOXP3 Monoclonal Antibody (PCH101), PE-Cyanine5, …
WebNov 10, 2024 · Foxp3 staining was performed using a Foxp3/Transcription Factor Staining Buffer Set from eBioscience with an anti-mouse Foxp3 antibody (clone MF-14) and anti-human Foxp3 antibody (clone 259D ... WebEach lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 10 6 cells in 100 µl volume. For Western blotting, the suggested working dilution(s) is ≤ 5.0 µg/ml in antibody dilution buffer. jim shore christmas angels 2022
PE anti-human FOXP3 Antibody anti-FOXP3 - 259D - BioLegend
WebFlow Cyt (intra): HEK-293T transfected with a GFP-tagged FOXP3 expression construct. IP: HEK-293T transfected with a GFP-tagged FOXP3 expression construct whole cell lysate. General notes This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility WebResuspend cells in fluorochrome-conjugated secondary antibody, diluted in 1X FoxP3/Transcription Factor Permeabilization Buffer at the recommended dilution. Incubate for 30 min at room temperature (20-25°C). Protect from light. Wash by centrifugation in excess 1X FoxP3/Transcription Factor Permeabilization Buffer. Discard supernatant. … WebFlow Cytometry (Intracellular) - FITC Anti-FOXP3 antibody [3G3] (ab210230) Intracellular flow cytometric analysis of Mouse C57Bl/6 splenocytes stained with APC Anti-Mouse CD4, followed by intracellular labeling of Foxp3 with ab210230 at 0.015 ug/test (right panel) or FITC Mouse IgG1 isotype control at 0.015 ug/test (left panel). instant checkmate + jignashu bodawala